BibTeX Entry


@article{YoussefEtAl:Proteomics23,
  author	= {Youssef, Ahmed and Bian, Fei and Havugimana, Pierre and Crovella, Mark and Emili, Andrew},
  title		= {Dynamic Remodeling of Escherichia coli Interactome in Response to Environmental Perturbations},
  journal	= {Proteomics},
  year		= {2023},
  URL		= {http://www.cs.bu.edu/faculty/crovella/paper-archive/Proteomics-2023-Youssef-Dynamic-remodeling.pdf},
  abstract	= {Proteins play an essential role in the vital biological processes governing cellular functions. Most proteins function as members of macromolecular machines, with the network of interacting proteins revealing the molecular mechanisms driving the formation of these complexes. Profiling the physiology-driven remodeling of these interactions within different contexts constitutes a crucial component to achieving a comprehensive systems-level understanding of interactome dynamics. Here, we apply co-fractionation mass spectrometry and computational modeling to quantify and profile the interactions of ~2,000 proteins in the bacterium Escherichia coli cultured under ten distinct culture conditions. The resulting quantitative co-elution patterns revealed large-scale condition-dependent interaction remodeling among protein complexes involved in diverse biochemical pathways in response to the unique environmental challenges. Network-level analysis highlighted interactome-wide biophysical properties and structural patterns governing interaction remodeling. Our results provide evidence of the local and global plasticity of the E. coli interactome along with a rigorous generalizable framework to define protein interaction specificity. We provide an accompanying interactive web application to facilitate exploration of these rewired networks.},
  doi		= {10.1002/pmic.202200404},
}